Multiview Imaging with Real-Time Microwave Camera from Known Positions

IEEE International Symposium on Antennas and Propagation and USNC-URSI Radio Science Meeting (2018, Boston

Evaluation of Positioning Techniques for mm-Wave Portable Scanners

European Conference on Antennas and Propagation (12th, 2018, London

Passive RFID System for Object Shape Estimation

European Conference on Antennas and Propagation (12th, 2018, London

Terasense WP03 radiation and sensor measurement lab workpackage

This paper explains the progress accomplished in the WP03 of the Terasense Project (TERAHERTZ TECHNOLOGY FOR ELECTROMAGNETIC SENSING APPLICATIONS) approved in the 2008 CONSOLIDERINGENIO program (project CSD2008-0068). The Radiation and Sensor Measurement Lab (RSMLab) is a laboratory based in the existing antenna measurement laboratories at UPM, UC3 and UNiOvi and the new capacities to extend the measurement range from the millimetre wave to the THz region. This laboratory is intended to be shared in more than one place and with more than one institution, in such a way that we could take advantage of other research financial sources and contributions from other institutions with interest in the same field of measurements. One important task will be the international links between the RSMLab and other European and international institutions dedicated to the antenna and sensor measurement in the same frequency range

Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)

In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. For example, a key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process versus those that measure fl ux through the autophagy pathway (i.e., the complete process including the amount and rate of cargo sequestered and degraded). In particular, a block in macroautophagy that results in autophagosome accumulation must be differentiated from stimuli that increase autophagic activity, defi ned as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (inmost higher eukaryotes and some protists such as Dictyostelium ) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the fi eld understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. It is worth emphasizing here that lysosomal digestion is a stage of autophagy and evaluating its competence is a crucial part of the evaluation of autophagic flux, or complete autophagy. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. Along these lines, because of the potential for pleiotropic effects due to blocking autophagy through genetic manipulation it is imperative to delete or knock down more than one autophagy-related gene. In addition, some individual Atg proteins, or groups of proteins, are involved in other cellular pathways so not all Atg proteins can be used as a specific marker for an autophagic process. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field